![]() ![]() Therefore, DZ-loaded cubogel could be considered as promising delivery system to boost the transcorneal permeation hence corneal bioavailability of DZ as antiglaucomal drug.Ĭubosomes are thermodynamically stable self-assembled nanoparticles cubic liquid crystalline phases. h/ml) was attained from cubogel compared to the market product Trusopt ® eye drops (115.40 mg. CLSM showed a deeper penetration of more than 2.5-fold. Results revealed that the optimized cubogel was considerably safe, stable and competent to corneal delivery as assured by draize and histological examination. Moreover, in vivo studies of the optimized cubogel include draize test, histological examination, confocal laser scanning microscopy (CLSM) and intraocular pressure (IOP) measurement. Carbopol 934 (1% w/v) as gelling agent was used to prepare the optimum cubogel, which was further evaluated by DSC, ex-vivo permeation and stability studies at 4 ☌ for three months. Results revealed that the optimum formula showed PS of 153.3 ± 8.4 n, Zp of 32 ± 3 −mV and 37.78 ± 1.3% released after 2 h. The optimized formulation composed of: 6.13% w/w of MO, 0.75% w/w of F127 and prepared at 1200 rpm stirring speed was chosen based on the criteria of minimum PS (nm), maximum Zp (−mV) and minimum Q 2 h (%). ![]() The prepared formulae were characterized via drug content DC (%), particle size PS (nm), polydispersity index PDI, zeta potential Zp (−mV), in-vitro drug release (Q 2 h%) and finally TEM. ![]() DZ-loaded cubosomal dispersions were prepared according to Box-Behnken design, where the effect of independent variables Monoolein MO concentration (2.5, 5 and 7.5%w/w), Pluronic ® F127 concentration (0.25, 0.5 and 0.75%w/w) and magnetic stirrer speed of (400, 8 rpm) was studied on PS (nm), Zp (−mV) and Q 2 h (%) respectively. The aim of this work is to survey the potential of cubogel as an ocular dosage form to boost the corneal permeability of Dorzolamide Hydrochloride DZ an antiglaucomal drug. ![]()
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